Detection Kit for SARS-CoV-2 RNA Presence in Biological Material Using Real_Time SmartAmp Method is intended for SARS-CoV-2 coronavirus genome RNA extraction from nasopharyngeal/oropharyngeal swabs and sputum from patients.
1. Reagent Е, transparent colorless liquid.
2. Reagent P, transparent colorless liquid.
3. PC (positive control sample), transparent colorless liquid.
4. NC (negative control sample), transparent colorless liquid.
12 months from the production date.
Do not use the expired Detection Kit.
Store at t -80°С…-20°С
Before starting the reaction, defrost the reagents on dry ice.
1. Dispense 6 μl from the Reagent E tube into each test tube or each amplifier microtube.
2. Mix 10 μl of the RNA extracted from the patient sample with 4 μl from the Reagent P tube.
3. Add a mixed sample (14 μl) to each test tube with Reagent E.
4. Dispense 6 μl from the Reagent E tube into each test tube or each amplifier microtube.
5. Mix 10 μl from the PC tube with 4 μl from the Reagent P tube.
6. Add a mixed sample (14 μl) to each test tube with Reagent E.
7. Dispense 6 μl from the Reagent E tube into each test tube or each amplifier microtube.
8. Mix 10 μl from the NC tube containing DNase/RNase-Free Distilled Water with 4 μl from the Reagent P tube.
9. Add a mixed sample (14 μl) to each test tube with Reagent E.